The success rates in IVF treatment are higher than before. New methods are also effective in increasing this ratio. Yeditepe University Hospital IVF Center Director Dr. We talked with Cem Fıçıcıoğlu about factors that increase success in IVF treatment.
What is the assisted hatching method?
Another procedure applied to the embryo in IVF treatment is assisted hatching. There is a protective membrane called “Zona Pellucida us around the embryo. After the embryo is introduced into the uterus, it continues to divide and grow, and when it reaches a certain size, it tears the membrane and clings into the uterus. Before the embryo is transferred, it is called Assisted Hatching, whereby the outer membrane surrounding it is mechanically thinned using an acid tyrode or laser-assisted, or completely punctured. In our center, the laser method, which is a very reliable and useful technique, has a very short application time. This process; If the membrane of the embryo is thick (over 15 microns), it is used in women over 35 years of age, in cases where pregnancy did not occur despite the transfer of good embryos in previous applications and in the transfer of frozen embryos.
Are laser treatments applied only to embryos from older women?
The budding applications with the help of laser are performed in cases where the layer of shingles around the egg is thicker than normal regardless of the age of the female spouse and in cases where the pregnancy cannot be achieved despite the application of more than one IVF or microinjection. There are a large number of cases that conceive and have healthy children in the third, fourth line and ninth application with laser-assisted budding applications. In addition to assisted budding, the laser is also used to improve the quality of embryos transferred to the expectant mother by removing toxic waste from the embryo.
What is blastocyst transfer?
The fertilized egg is divided and becomes 2-4 cells on day 2 and in classical in vitro fertilization, the embryo in this stage is transferred to the mother's womb. In natural pregnancies, the embryo reaches the uterus on the 5th day in the multicellular stage called the balstocyst. The embryo at this stage has a higher chance of holding onto the uterus. Today, it is possible to develop embryos until the blastocyst period with the special media developed. Pregnancy rates have been increased with blastocyst transfer. In the world, the first consecutive transfer application in Singapore, the embryo transfer to the mother on the second day after the embryo transfer continues to develop in the special embryos and blastocyst stage of the embryo transfer can be obtained on the 6th day of embryo transfer. Pregnancy rates increase significantly with sequential transfer and the abortion rate decreases very much in the pregnancies obtained by balstocyst transfer.
What is co-culture?
In order to provide better embryo development in this group of patients, a supporting culture medium (co-culture) prepared from endometrium cells is used instead of synthetic fattening solutions. Your period is 20-23. Cells taken from the uterus by biopsy between the days of the culture medium is prepared by reproduction in the laboratory and embryo development is continued in this environment. With this method, embryos are grown on uterine tissue similar to those in the body and the growth factors in the medium support embryo development. With this method, embryo development of better quality is achieved and pregnancy rates are increased in patients compared to previous trials.